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Historically, the estuary has been affected by urban, industrial, agricultural and harbor activities, and occasional accidents. The present study sought to investigate the pollution threat to aquatic organisms in order to evaluate the potential effects of pollutants.
Among the biomarkers, the somatic index, chemical analysis of bile, biochemical, genetic and morphological parameters were considered. Chemical analysis of bile showed a continuous bioavailability of polycyclic aromatic hydrocarbons PAHs according to proximity to the harbor site.
The histopathological findings have demonstrated aconsiderable incidence of severe pathologies in the liver and gills, corroborated by biochemical disturbances and genetic damage. According to Vanparys et al. In general, marine biota is exposed to a variety of contaminants, and biomarkers are useful to verify their biological effects and establish some of the aforementioned links.
Viarengo et al , for example, described the importance of biomarkers at different levels of biological organization in bio monitoring studies, and Pikkarainen reported that the necessity of monitoring biological effects has been recognized by several international programs and committees HELCOM, ; ICES, ; UNEP, Biomarkers may be derived from morphological, biochemical and physiological alterations in organisms on their exposure to xenobiotics, integrating biological responses to contamination and may thereby indicate sublethal exposure.
According to Lyons et al. Fish are particularly sensitive to the environmental contamination of water and, therefore, pollutants may significantly interfere with several of their biochemical processes.